Abstract
We present here a viscosity sensitive fluorescent dye, namely thiophene dihemicyanine (TDHC), that enables the specific staining of mitochondria. In comparison to the common mitochondria tracker (Mitotracker Deep Red, MTDR), this dye demonstrated its unique ability for robust staining of mitochondria with high photostability and ultrahigh signal-to-noise ratio (SNR). Moreover, TDHC also showed high sensitivity towards mitochondria membrane potential (ΔΨm) and intramitochondria viscosity change. Consequently, this dye was utilized in real-time monitoring of mitochondria transport in primary cortical neurons. Finally, the Two-Photon Microscopy (TPM) imaging ability of TDHC was also demonstrated.
Original language | English |
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Pages (from-to) | 885-891 |
Number of pages | 7 |
Journal | Biosensors and Bioelectronics |
Volume | 86 |
DOIs | |
State | Published - 15 Dec 2016 |
Bibliographical note
Funding Information:This study was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIP) (No. 2012R1A3A2048814 ). Mass spectral data were obtained from the Korea Basic Science Institute (Daegu) on a Jeol JMS 700 high resolution mass spectrometer. National Research Foundation (NRF) grants funded by the Korean Government ( 2012R1A2A1A03670456 ) are acknowledged.
Publisher Copyright:
© 2016 Elsevier B.V.
Keywords
- Fluorescent probe
- Mitochondria selective probe
- Mitochondria transport in neurons
- Two photon probe
- Viscosity probe