A viscosity sensitive fluorescent dye for real-time monitoring of mitochondria transport in neurons

Yeonju Baek, Sang Jun Park, Xin Zhou, Gyungmi Kim, Hwan Myung Kim, Juyoung Yoon

Research output: Contribution to journalArticlepeer-review

82 Scopus citations

Abstract

We present here a viscosity sensitive fluorescent dye, namely thiophene dihemicyanine (TDHC), that enables the specific staining of mitochondria. In comparison to the common mitochondria tracker (Mitotracker Deep Red, MTDR), this dye demonstrated its unique ability for robust staining of mitochondria with high photostability and ultrahigh signal-to-noise ratio (SNR). Moreover, TDHC also showed high sensitivity towards mitochondria membrane potential (ΔΨm) and intramitochondria viscosity change. Consequently, this dye was utilized in real-time monitoring of mitochondria transport in primary cortical neurons. Finally, the Two-Photon Microscopy (TPM) imaging ability of TDHC was also demonstrated.

Original languageEnglish
Pages (from-to)885-891
Number of pages7
JournalBiosensors and Bioelectronics
Volume86
DOIs
StatePublished - 15 Dec 2016

Keywords

  • Fluorescent probe
  • Mitochondria selective probe
  • Mitochondria transport in neurons
  • Two photon probe
  • Viscosity probe

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