Abstract
In this study, an LC method for the simultaneous determination of six bioactive compounds from Angelica tenuissima, namely chlorogenic acid, ferulic acid, Z-ligustilide, nodakenin, decursin and decursinol angelate was developed and validated. Chromatographic analysis was carried out on a C18 column with a mobile phase consisting of 0.1% formic acid, methanol and acetonitrile at a flow rate of 0.8 mL min-1 and the effluent from the column was monitored by UV detector at 325 nm. The excellent linear behavior was observed over the investigated concentration range for reported compounds. The intra-and inter-day precision over the concentration range of compounds were lower than 1.7% (as relative standard deviation), and accuracy was between 97.2 and 106.0%. These results showed that the developed method is accurate, reproducible, and consequently applicable for the quantitation of bioactive components from the ethanolic extract of Angelica tenuissima Nakai
| Original language | English |
|---|---|
| Pages (from-to) | 1079-1085 |
| Number of pages | 7 |
| Journal | Chromatographia |
| Volume | 70 |
| Issue number | 7-8 |
| DOIs | |
| State | Published - Oct 2009 |
Bibliographical note
Funding Information:This work was supported in part by grants from Korea Food and Drug Administration and in part by Seoul Research and Business Development Program.
Keywords
- Angelica tenuissima
- Column liquid chromatography
- Coumarins and phthalides
- Phenolic acids