A Two-Photon Fluorescent Probe for Imaging Endogenous ONOO- near NMDA Receptors in Neuronal Cells and Hippocampal Tissues

Dayoung Lee, Chang Su Lim, Gyeongju Ko, Dayoung Kim, Myoung Ki Cho, Sang Jip Nam, Hwan Myung Kim, Juyoung Yoon

Research output: Contribution to journalArticlepeer-review

57 Scopus citations

Abstract

In this study, we developed a two-photon fluorescent probe for detection of peroxynitrite (ONOO-) near the N-methyl-d-aspartate (NMDA) receptor. This naphthalimide-based probe contains a boronic acid reactive group and an ifenprodil-like tail, which serves as an NMDA receptor targeting unit. The probe displays high sensitivity and selectivity, along with a fast response time in aqueous solution. More importantly, the probe can be employed along with two-photon fluorescence microscopy to detect endogenous ONOO- near NMDA receptors in neuronal cells as well as in hippocampal tissues. The results suggest that the probe has the potential of serving as a useful imaging tool for studying ONOO- related diseases in the nervous system.

Original languageEnglish
Pages (from-to)9347-9352
Number of pages6
JournalAnalytical Chemistry
Volume90
Issue number15
DOIs
StatePublished - 7 Aug 2018

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