A two-photon fluorescent probe for colorimetric and ratiometric monitoring of mercury in live cells and tissues

Liyan Chen; Sang Jun Park; Di Wu; Hwan Myung Kim; Juyoung Yoon

doi:10.1039/c8cc08608g

A two-photon fluorescent probe for colorimetric and ratiometric monitoring of mercury in live cells and tissues

Liyan Chen, Sang Jun Park, Di Wu, Hwan Myung Kim, Juyoung Yoon

Department of Chemistry & Nanoscience

Research output: Contribution to journal › Article › peer-review

94 Scopus citations

Abstract

Owing to the extreme toxicity of mercury, methods for its selective and sensitive sensing in solutions, and in live cells and tissues are in great demand. In this study, we developed a naphthalimide-based diphenylphosphinothioyl group-containing fluorescent and colorimetric probe that selectively detects mercury (Hg²⁺). Upon addition of mercury (Hg²⁺) to a solution of the probe, both a colorimetric change from colorless to yellow and a fluorescence change from blue to green (under a 365 nm hand-held UV lamp) occur, both of which can be observed using the "naked-eye". Furthermore, the probe possesses the capability of sensing intracellular mercury in both live cells and tissues using dual-emission channels and two-photon microscopy.

Original language	English
Pages (from-to)	1766-1769
Number of pages	4
Journal	Chemical Communications
Volume	55
Issue number	12
DOIs	https://doi.org/10.1039/c8cc08608g
State	Published - 2019

Bibliographical note

Funding Information:
This study was supported financially by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (No. 2012R1A3A2048814 for J. Yoon and No. 2016R1E1A1A02920873 for H. M. Kim). The Korea Basic Science Institute (Western Seoul) is acknowledged for the LC/MS data. FAB mass spectral data were obtained from the Korea Basic Science Institute (Daegu) on a Jeol JMS 700 high resolution mass spectrometer.

Publisher Copyright:
© 2019 The Royal Society of Chemistry.

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title = "A two-photon fluorescent probe for colorimetric and ratiometric monitoring of mercury in live cells and tissues",

abstract = "Owing to the extreme toxicity of mercury, methods for its selective and sensitive sensing in solutions, and in live cells and tissues are in great demand. In this study, we developed a naphthalimide-based diphenylphosphinothioyl group-containing fluorescent and colorimetric probe that selectively detects mercury (Hg2+). Upon addition of mercury (Hg2+) to a solution of the probe, both a colorimetric change from colorless to yellow and a fluorescence change from blue to green (under a 365 nm hand-held UV lamp) occur, both of which can be observed using the {"}naked-eye{"}. Furthermore, the probe possesses the capability of sensing intracellular mercury in both live cells and tissues using dual-emission channels and two-photon microscopy.",

author = "Liyan Chen and Park, {Sang Jun} and Di Wu and Kim, {Hwan Myung} and Juyoung Yoon",

note = "Funding Information: This study was supported financially by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (No. 2012R1A3A2048814 for J. Yoon and No. 2016R1E1A1A02920873 for H. M. Kim). The Korea Basic Science Institute (Western Seoul) is acknowledged for the LC/MS data. FAB mass spectral data were obtained from the Korea Basic Science Institute (Daegu) on a Jeol JMS 700 high resolution mass spectrometer. Publisher Copyright: {\textcopyright} 2019 The Royal Society of Chemistry.",

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doi = "10.1039/c8cc08608g",

language = "English",

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pages = "1766--1769",

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T1 - A two-photon fluorescent probe for colorimetric and ratiometric monitoring of mercury in live cells and tissues

AU - Chen, Liyan

AU - Park, Sang Jun

AU - Wu, Di

AU - Kim, Hwan Myung

AU - Yoon, Juyoung

N1 - Funding Information: This study was supported financially by the National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIP) (No. 2012R1A3A2048814 for J. Yoon and No. 2016R1E1A1A02920873 for H. M. Kim). The Korea Basic Science Institute (Western Seoul) is acknowledged for the LC/MS data. FAB mass spectral data were obtained from the Korea Basic Science Institute (Daegu) on a Jeol JMS 700 high resolution mass spectrometer. Publisher Copyright: © 2019 The Royal Society of Chemistry.

PY - 2019

Y1 - 2019

N2 - Owing to the extreme toxicity of mercury, methods for its selective and sensitive sensing in solutions, and in live cells and tissues are in great demand. In this study, we developed a naphthalimide-based diphenylphosphinothioyl group-containing fluorescent and colorimetric probe that selectively detects mercury (Hg2+). Upon addition of mercury (Hg2+) to a solution of the probe, both a colorimetric change from colorless to yellow and a fluorescence change from blue to green (under a 365 nm hand-held UV lamp) occur, both of which can be observed using the "naked-eye". Furthermore, the probe possesses the capability of sensing intracellular mercury in both live cells and tissues using dual-emission channels and two-photon microscopy.

AB - Owing to the extreme toxicity of mercury, methods for its selective and sensitive sensing in solutions, and in live cells and tissues are in great demand. In this study, we developed a naphthalimide-based diphenylphosphinothioyl group-containing fluorescent and colorimetric probe that selectively detects mercury (Hg2+). Upon addition of mercury (Hg2+) to a solution of the probe, both a colorimetric change from colorless to yellow and a fluorescence change from blue to green (under a 365 nm hand-held UV lamp) occur, both of which can be observed using the "naked-eye". Furthermore, the probe possesses the capability of sensing intracellular mercury in both live cells and tissues using dual-emission channels and two-photon microscopy.

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VL - 55

SP - 1766

EP - 1769

JO - Chemical Communications

JF - Chemical Communications

IS - 12

ER -

A two-photon fluorescent probe for colorimetric and ratiometric monitoring of mercury in live cells and tissues

Abstract

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