@article{e0e4e1444d6046238f326ec2b43b0ad8,
title = "A solution to the biophysical fractionation of extracellular vesicles: Acoustic Nanoscale Separation via Wave-pillar Excitation Resonance (ANSWER)",
abstract = "High-precision isolation of small extracellular vesicles (sEVs) from biofluids is essential toward developing next-generation liquid biopsies and regenerative therapies. However, current methods of sEV separation require specialized equipment and time-consuming protocols and have difficulties producing highly pure subpopulations of sEVs. Here, we present Acoustic Nanoscale Separation via Wave-pillar Excitation Resonance (ANSWER), which allows single-step, rapid (<10 min), high-purity (>96% small exosomes, >80% exomeres) fractionation of sEV subpopulations from biofluids without the need for any sample preprocessing. Particles are iteratively deflected in a size-selective manner via an excitation resonance. This previously unidentified phenomenon generates patterns of virtual, tunable, pillar-like acoustic field in a fluid using surface acoustic waves. Highly precise sEV fractionation without the need for sample preprocessing or complex nanofabrication methods has been demonstrated using ANSWER, showing potential as a powerful tool that will enable more in-depth studies into the complexity, heterogeneity, and functionality of sEV subpopulations.",
author = "Jinxin Zhang and Chuyi Chen and Ryan Becker and Joseph Rufo and Shujie Yang and John Mai and Peiran Zhang and Yuyang Gu and Zeyu Wang and Zhehan Ma and Jianping Xia and Nanjing Hao and Zhenhua Tian and Wong, {David T.W.} and Yoel Sadovsky and Lee, {Luke P.} and Huang, {Tony Jun}",
note = "Funding Information: Acknowledgments Funding: W e acknowledge support from the National Institutes of Health (grant nos. R01HD103727,UH3TR002978,U18TR003778,R01GM132603,R01GM143439,R01GM135486, R01GM144417,andR44AG063643)andNationalScienceFoundation(grantnos.CMMI-2104295andCMMI-2104526).Authorcontributions:J.Z.developedthesy stemconceptand ledtheexperimentalwork.L.P .L. andT .J.H. guidedtheexperimentaldesign,figuredrawing,and paperwriting.J.Z.,C.C.,J.X.,andY .G. conductedthetheoreticalandnumericalanalyses.Z.W ., Z.M.,N.H.,andD.T .W .W .contributedtobiologicalsamplepreparationandprocess.J.Z.,P .Z., S.Y ., andZ.T .contributedtothedevicefabricationandimprovement.P .Z., Z.T ., S.Y ., D.T .W .W ., Y .S., L.P .L., andT .J.H. pro vided guidanceandcontributedtothedesignandanalysisthroughoutthe project.ThemanuscriptwaswrittenbyJ.Z.,J.M.,C.C.,P .Z., J.R.,R.B.,Y .S., L.P .L., andT .J.H. Competinginterests:T .J.H. hascofoundedastartupcompany,AscentBio-NanoT echnologies Inc.,tocommercializetechnologiesinvolvingacoustofluidicsandacoustictweezers.D.T .W .W . hasequityinLiquidDiagnostics.Theotherauthorsdeclarethattheyhav enocompeting interests.Dataandmaterialsavailability:Alldataneededtoevaluatetheconclusionsinthe paperarepresentinthepaperand/ortheSupplementaryMaterials. Funding Information: Funding: We acknowledge support from the National Institutes of Health (grant nos. R01HD103727, UH3TR002978, U18TR003778, R01GM132603, R01GM143439, R01GM135486, R01GM144417, and R44AG063643) and National Science Foundation (grant nos. CMMI-2104295 and CMMI-2104526). Publisher Copyright: {\textcopyright} 2022 American Association for the Advancement of Science. All rights reserved.",
year = "2022",
month = nov,
doi = "10.1126/sciadv.ade0640",
language = "English",
volume = "8",
journal = "Science Advances",
issn = "2375-2548",
publisher = "American Association for the Advancement of Science",
number = "47",
}