A simple and efficient strategy for the sensitivity enhancement of DNA hybridization based on the coupling between propagating and localized surface plasmons

Kyungwha Chung; Jieun Lee; Ji Eun Lee; Ji Yong Lee; Seyoung Moon; King Hang Aaron Lau; Donghyun Kim; Dong Ha Kim

doi:10.1016/j.snb.2012.10.025

A simple and efficient strategy for the sensitivity enhancement of DNA hybridization based on the coupling between propagating and localized surface plasmons

Kyungwha Chung, Jieun Lee, Ji Eun Lee, Ji Yong Lee, Seyoung Moon, King Hang Aaron Lau, Donghyun Kim, Dong Ha Kim

Department of Chemistry & Nanoscience

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

This study demonstrates a simple and efficient strategy for the sensitivity improvement in detection of biomolecules via surface plasmon resonance field enhancement in a Kretschmann configuration. Synergistic coupling effects between propagating and localized surface plasmons (SPs) were demonstrated by incorporating arrays of Au nanoparticles (AuNPs) in-between DNA sensing assays. AuNPs with 5 nm or 15 nm diameter were incorporated respectively on the top of the streptavidin layer, and the couplinginduced sensitivity enhancement was systematically investigated. Uniform dispersion of AuNPs was confirmed by TEM analysis. The overall sensing capability of each system was investigated in terms of the reflectivity change, angular shift, and affinity constant for the DNA hybridization process and it was found that AuNP-arrays with appropriate size and lateral distribution led to the best efficiency. The experimental results were in good agreement with numerical simulation data based on a rigorous coupled wave analysis (RCWA).

Original language	English
Pages (from-to)	1074-1080
Number of pages	7
Journal	Sensors and Actuators, B: Chemical
Volume	176
DOIs	https://doi.org/10.1016/j.snb.2012.10.025
State	Published - 2013

Bibliographical note

Funding Information:
This work was supported by National Research Foundation of Korea Grant funded by the Korean Government ( 2012047815 , 2012K001319 ) and by the Ewha Global Top5 Grant 2012 of Ewha Womans University.

Keywords

Au nanoparticle
Colloidal Au-amplified SPR
DNA hybridization
Plasmonic coupling
SPR sensor

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10.1016/j.snb.2012.10.025

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title = "A simple and efficient strategy for the sensitivity enhancement of DNA hybridization based on the coupling between propagating and localized surface plasmons",

abstract = "This study demonstrates a simple and efficient strategy for the sensitivity improvement in detection of biomolecules via surface plasmon resonance field enhancement in a Kretschmann configuration. Synergistic coupling effects between propagating and localized surface plasmons (SPs) were demonstrated by incorporating arrays of Au nanoparticles (AuNPs) in-between DNA sensing assays. AuNPs with 5 nm or 15 nm diameter were incorporated respectively on the top of the streptavidin layer, and the couplinginduced sensitivity enhancement was systematically investigated. Uniform dispersion of AuNPs was confirmed by TEM analysis. The overall sensing capability of each system was investigated in terms of the reflectivity change, angular shift, and affinity constant for the DNA hybridization process and it was found that AuNP-arrays with appropriate size and lateral distribution led to the best efficiency. The experimental results were in good agreement with numerical simulation data based on a rigorous coupled wave analysis (RCWA).",

keywords = "Au nanoparticle, Colloidal Au-amplified SPR, DNA hybridization, Plasmonic coupling, SPR sensor",

author = "Kyungwha Chung and Jieun Lee and Lee, {Ji Eun} and Lee, {Ji Yong} and Seyoung Moon and Lau, {King Hang Aaron} and Donghyun Kim and Kim, {Dong Ha}",

note = "Funding Information: This work was supported by National Research Foundation of Korea Grant funded by the Korean Government ( 2012047815 , 2012K001319 ) and by the Ewha Global Top5 Grant 2012 of Ewha Womans University. ",

year = "2013",

doi = "10.1016/j.snb.2012.10.025",

language = "English",

volume = "176",

pages = "1074--1080",

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T1 - A simple and efficient strategy for the sensitivity enhancement of DNA hybridization based on the coupling between propagating and localized surface plasmons

AU - Chung, Kyungwha

AU - Lee, Jieun

AU - Lee, Ji Eun

AU - Lee, Ji Yong

AU - Moon, Seyoung

AU - Lau, King Hang Aaron

AU - Kim, Donghyun

AU - Kim, Dong Ha

N1 - Funding Information: This work was supported by National Research Foundation of Korea Grant funded by the Korean Government ( 2012047815 , 2012K001319 ) and by the Ewha Global Top5 Grant 2012 of Ewha Womans University.

PY - 2013

Y1 - 2013

N2 - This study demonstrates a simple and efficient strategy for the sensitivity improvement in detection of biomolecules via surface plasmon resonance field enhancement in a Kretschmann configuration. Synergistic coupling effects between propagating and localized surface plasmons (SPs) were demonstrated by incorporating arrays of Au nanoparticles (AuNPs) in-between DNA sensing assays. AuNPs with 5 nm or 15 nm diameter were incorporated respectively on the top of the streptavidin layer, and the couplinginduced sensitivity enhancement was systematically investigated. Uniform dispersion of AuNPs was confirmed by TEM analysis. The overall sensing capability of each system was investigated in terms of the reflectivity change, angular shift, and affinity constant for the DNA hybridization process and it was found that AuNP-arrays with appropriate size and lateral distribution led to the best efficiency. The experimental results were in good agreement with numerical simulation data based on a rigorous coupled wave analysis (RCWA).

AB - This study demonstrates a simple and efficient strategy for the sensitivity improvement in detection of biomolecules via surface plasmon resonance field enhancement in a Kretschmann configuration. Synergistic coupling effects between propagating and localized surface plasmons (SPs) were demonstrated by incorporating arrays of Au nanoparticles (AuNPs) in-between DNA sensing assays. AuNPs with 5 nm or 15 nm diameter were incorporated respectively on the top of the streptavidin layer, and the couplinginduced sensitivity enhancement was systematically investigated. Uniform dispersion of AuNPs was confirmed by TEM analysis. The overall sensing capability of each system was investigated in terms of the reflectivity change, angular shift, and affinity constant for the DNA hybridization process and it was found that AuNP-arrays with appropriate size and lateral distribution led to the best efficiency. The experimental results were in good agreement with numerical simulation data based on a rigorous coupled wave analysis (RCWA).

KW - Au nanoparticle

KW - Colloidal Au-amplified SPR

KW - DNA hybridization

KW - Plasmonic coupling

KW - SPR sensor

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DO - 10.1016/j.snb.2012.10.025

M3 - Article

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SN - 0925-4005

VL - 176

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EP - 1080

JO - Sensors and Actuators, B: Chemical

JF - Sensors and Actuators, B: Chemical

ER -

A simple and efficient strategy for the sensitivity enhancement of DNA hybridization based on the coupling between propagating and localized surface plasmons

Abstract

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Keywords

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