@article{540928e53ff945ccb5121503053bd25d,
title = "A Selective Imidazoline-2-thione-Bearing Two-Photon Fluorescent Probe for Hypochlorous Acid in Mitochondria",
abstract = "Hypochlorite (OCl-) plays a key role in the immune system and is involved in various diseases. Accordingly, direct detection of endogenous OCl- at the subcellular level is important for understanding inflammation and cellular apoptosis. In the current study, a two-photon fluorescent off/on probe (PNIS) bearing imidazoline-2-thione as an OCl- recognition unit and triphenylphosphine (TPP) as a mitochondrial-targeting group was synthesized and examined for its ability to image mitochondrial OCl- in situ. This probe, based on the specific reaction between imidazoline-2-thione and OCl-, displayed a selective fluorescent off/on response to OCl- with the various reactive oxygen species in a physiological medium. PNIS was successfully applied to image of endogenously produced mitochondrial OCl- in live RAW 264.7 cells via two-photon microscopy.",
author = "Qingling Xu and Heo, {Cheol Ho} and Kim, {Jin A.} and Lee, {Hye Sue} and Ying Hu and Dayoung Kim and Swamy, {Kunemadihalli Mathada Kotraiah} and Gyoungmi Kim and Nam, {Sang Jip} and Kim, {Hwan Myung} and Juyoung Yoon",
note = "Funding Information: J.Y. acknowledges a grant from the National Creative Research Initiative program of the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP) (Grant 2012R1A3A2048814). H.M.K. acknowledges a grant from the National Research Foundation of Korea (NRF) funded by the Korean government (MSIP) (Grant 2012R1A2A1A03670456). S.N. acknowledges the National Research Council of Science and Technology through the Degree & Research Center program (Grant DRC-14-1-KBSI). Publisher Copyright: {\textcopyright} 2016 American Chemical Society.",
year = "2016",
month = jun,
day = "21",
doi = "10.1021/acs.analchem.6b01738",
language = "English",
volume = "88",
pages = "6615--6620",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "12",
}