TY - JOUR
T1 - A rhodamine-based fluorescent probe for the detection of lysosomal pH changes in living cells
AU - Lee, Dayoung
AU - Swamy, K. M.K.
AU - Hong, Joohee
AU - Lee, Songyi
AU - Yoon, Juyoung
N1 - Funding Information:
This research was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (National Creative Research Initiative: No. 2012R1A3A2048814 ) for J.Y. and the Ministry of Education (No. 2017R1A6A3A04004954 ) for S.L. Mass spectral data were obtained from the Korea Basic Science Institute (Daegu) on a Jeol JMS 700 high resolution mass spectrometer.
Funding Information:
This research was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (National Creative Research Initiative: No. 2012R1A3A2048814) for J.Y. and the Ministry of Education (No. 2017R1A6A3A04004954) for S.L. Mass spectral data were obtained from the Korea Basic Science Institute (Daegu) on a Jeol JMS 700 high resolution mass spectrometer.
Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2018/8/1
Y1 - 2018/8/1
N2 - Sensing and monitoring of pH changes in live cells are of great importance for understanding of cellular signaling responses. In this study, we developed a highly sensitive and selective pH sensor, rhodamine-based fluorescence dye 1. The fluorescent emission of 1 at 588 nm increased with the pH decreasing (from pH 7.0–4.0) due to the ring-opening of spirolactam by H+. 1 exhibited good water solubility, high quantum yield, and fast response to pH changes. Moreover, 1 can stain lysosomes in living cells with high photostability and low cytotoxicity. Importantly, 1 was used for monitoring pH changes in lysosomes induced by chloroquine, artesunate and dexamethasone. Therefore, our work provides the potential of probe 1 for visualizing the intracellular changes of pH in clinical and medical applications.
AB - Sensing and monitoring of pH changes in live cells are of great importance for understanding of cellular signaling responses. In this study, we developed a highly sensitive and selective pH sensor, rhodamine-based fluorescence dye 1. The fluorescent emission of 1 at 588 nm increased with the pH decreasing (from pH 7.0–4.0) due to the ring-opening of spirolactam by H+. 1 exhibited good water solubility, high quantum yield, and fast response to pH changes. Moreover, 1 can stain lysosomes in living cells with high photostability and low cytotoxicity. Importantly, 1 was used for monitoring pH changes in lysosomes induced by chloroquine, artesunate and dexamethasone. Therefore, our work provides the potential of probe 1 for visualizing the intracellular changes of pH in clinical and medical applications.
KW - Cell imaging
KW - Fluorescence
KW - Lysosome
KW - Rhodamine
KW - pH sensor
UR - http://www.scopus.com/inward/record.url?scp=85044607116&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2018.03.133
DO - 10.1016/j.snb.2018.03.133
M3 - Article
AN - SCOPUS:85044607116
SN - 0925-4005
VL - 266
SP - 416
EP - 421
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
ER -