A rhodamine-based fluorescent probe for the detection of lysosomal pH changes in living cells

Dayoung Lee, K. M.K. Swamy, Joohee Hong, Songyi Lee, Juyoung Yoon

Research output: Contribution to journalArticlepeer-review

89 Scopus citations

Abstract

Sensing and monitoring of pH changes in live cells are of great importance for understanding of cellular signaling responses. In this study, we developed a highly sensitive and selective pH sensor, rhodamine-based fluorescence dye 1. The fluorescent emission of 1 at 588 nm increased with the pH decreasing (from pH 7.0–4.0) due to the ring-opening of spirolactam by H+. 1 exhibited good water solubility, high quantum yield, and fast response to pH changes. Moreover, 1 can stain lysosomes in living cells with high photostability and low cytotoxicity. Importantly, 1 was used for monitoring pH changes in lysosomes induced by chloroquine, artesunate and dexamethasone. Therefore, our work provides the potential of probe 1 for visualizing the intracellular changes of pH in clinical and medical applications.

Original languageEnglish
Pages (from-to)416-421
Number of pages6
JournalSensors and Actuators, B: Chemical
Volume266
DOIs
StatePublished - 1 Aug 2018

Bibliographical note

Funding Information:
This research was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (National Creative Research Initiative: No. 2012R1A3A2048814 ) for J.Y. and the Ministry of Education (No. 2017R1A6A3A04004954 ) for S.L. Mass spectral data were obtained from the Korea Basic Science Institute (Daegu) on a Jeol JMS 700 high resolution mass spectrometer.

Funding Information:
This research was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (National Creative Research Initiative: No. 2012R1A3A2048814) for J.Y. and the Ministry of Education (No. 2017R1A6A3A04004954) for S.L. Mass spectral data were obtained from the Korea Basic Science Institute (Daegu) on a Jeol JMS 700 high resolution mass spectrometer.

Publisher Copyright:
© 2018 Elsevier B.V.

Keywords

  • Cell imaging
  • Fluorescence
  • Lysosome
  • Rhodamine
  • pH sensor

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