A rhodamine-based fluorescent probe for the detection of lysosomal pH changes in living cells

Dayoung Lee; K. M.K. Swamy; Joohee Hong; Songyi Lee; Juyoung Yoon

doi:10.1016/j.snb.2018.03.133

A rhodamine-based fluorescent probe for the detection of lysosomal pH changes in living cells

Dayoung Lee, K. M.K. Swamy, Joohee Hong, Songyi Lee, Juyoung Yoon

Department of Chemistry & Nanoscience

Research output: Contribution to journal › Article › peer-review

98 Scopus citations

Abstract

Sensing and monitoring of pH changes in live cells are of great importance for understanding of cellular signaling responses. In this study, we developed a highly sensitive and selective pH sensor, rhodamine-based fluorescence dye 1. The fluorescent emission of 1 at 588 nm increased with the pH decreasing (from pH 7.0–4.0) due to the ring-opening of spirolactam by H⁺. 1 exhibited good water solubility, high quantum yield, and fast response to pH changes. Moreover, 1 can stain lysosomes in living cells with high photostability and low cytotoxicity. Importantly, 1 was used for monitoring pH changes in lysosomes induced by chloroquine, artesunate and dexamethasone. Therefore, our work provides the potential of probe 1 for visualizing the intracellular changes of pH in clinical and medical applications.

Original language	English
Pages (from-to)	416-421
Number of pages	6
Journal	Sensors and Actuators, B: Chemical
Volume	266
DOIs	https://doi.org/10.1016/j.snb.2018.03.133
State	Published - 1 Aug 2018

Bibliographical note

Funding Information:
This research was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (National Creative Research Initiative: No. 2012R1A3A2048814 ) for J.Y. and the Ministry of Education (No. 2017R1A6A3A04004954 ) for S.L. Mass spectral data were obtained from the Korea Basic Science Institute (Daegu) on a Jeol JMS 700 high resolution mass spectrometer.

Funding Information:
This research was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (National Creative Research Initiative: No. 2012R1A3A2048814) for J.Y. and the Ministry of Education (No. 2017R1A6A3A04004954) for S.L. Mass spectral data were obtained from the Korea Basic Science Institute (Daegu) on a Jeol JMS 700 high resolution mass spectrometer.

Publisher Copyright:
© 2018 Elsevier B.V.

Keywords

Cell imaging
Fluorescence
Lysosome
Rhodamine
pH sensor

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title = "A rhodamine-based fluorescent probe for the detection of lysosomal pH changes in living cells",

abstract = "Sensing and monitoring of pH changes in live cells are of great importance for understanding of cellular signaling responses. In this study, we developed a highly sensitive and selective pH sensor, rhodamine-based fluorescence dye 1. The fluorescent emission of 1 at 588 nm increased with the pH decreasing (from pH 7.0–4.0) due to the ring-opening of spirolactam by H+. 1 exhibited good water solubility, high quantum yield, and fast response to pH changes. Moreover, 1 can stain lysosomes in living cells with high photostability and low cytotoxicity. Importantly, 1 was used for monitoring pH changes in lysosomes induced by chloroquine, artesunate and dexamethasone. Therefore, our work provides the potential of probe 1 for visualizing the intracellular changes of pH in clinical and medical applications.",

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author = "Dayoung Lee and Swamy, {K. M.K.} and Joohee Hong and Songyi Lee and Juyoung Yoon",

note = "Funding Information: This research was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (National Creative Research Initiative: No. 2012R1A3A2048814 ) for J.Y. and the Ministry of Education (No. 2017R1A6A3A04004954 ) for S.L. Mass spectral data were obtained from the Korea Basic Science Institute (Daegu) on a Jeol JMS 700 high resolution mass spectrometer. Funding Information: This research was supported by the National Research Foundation of Korea (NRF) grant funded by the Korea government (National Creative Research Initiative: No. 2012R1A3A2048814) for J.Y. and the Ministry of Education (No. 2017R1A6A3A04004954) for S.L. Mass spectral data were obtained from the Korea Basic Science Institute (Daegu) on a Jeol JMS 700 high resolution mass spectrometer. Publisher Copyright: {\textcopyright} 2018 Elsevier B.V.",

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AU - Yoon, Juyoung

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N2 - Sensing and monitoring of pH changes in live cells are of great importance for understanding of cellular signaling responses. In this study, we developed a highly sensitive and selective pH sensor, rhodamine-based fluorescence dye 1. The fluorescent emission of 1 at 588 nm increased with the pH decreasing (from pH 7.0–4.0) due to the ring-opening of spirolactam by H+. 1 exhibited good water solubility, high quantum yield, and fast response to pH changes. Moreover, 1 can stain lysosomes in living cells with high photostability and low cytotoxicity. Importantly, 1 was used for monitoring pH changes in lysosomes induced by chloroquine, artesunate and dexamethasone. Therefore, our work provides the potential of probe 1 for visualizing the intracellular changes of pH in clinical and medical applications.

AB - Sensing and monitoring of pH changes in live cells are of great importance for understanding of cellular signaling responses. In this study, we developed a highly sensitive and selective pH sensor, rhodamine-based fluorescence dye 1. The fluorescent emission of 1 at 588 nm increased with the pH decreasing (from pH 7.0–4.0) due to the ring-opening of spirolactam by H+. 1 exhibited good water solubility, high quantum yield, and fast response to pH changes. Moreover, 1 can stain lysosomes in living cells with high photostability and low cytotoxicity. Importantly, 1 was used for monitoring pH changes in lysosomes induced by chloroquine, artesunate and dexamethasone. Therefore, our work provides the potential of probe 1 for visualizing the intracellular changes of pH in clinical and medical applications.

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A rhodamine-based fluorescent probe for the detection of lysosomal pH changes in living cells

Abstract

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Keywords

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