A Reversible Fluorescent Probe for Real-Time Quantitative Monitoring of Cellular Glutathione

Zhixue Liu; Xin Zhou; Yu Miao; Ying Hu; Nahyun Kwon; Xue Wu; Juyoung Yoon

doi:10.1002/anie.201702114

A Reversible Fluorescent Probe for Real-Time Quantitative Monitoring of Cellular Glutathione

Zhixue Liu, Xin Zhou, Yu Miao, Ying Hu, Nahyun Kwon, Xue Wu, Juyoung Yoon

Department of Chemistry & Nanoscience

Research output: Contribution to journal › Article › peer-review

282 Scopus citations

Abstract

The ability to monitor and quantify glutathione (GSH) in live cells is essential in order to gain a detailed understanding of GSH-related pathological events. However, owing to their irreversible response mechanisms, most existing fluorescent GSH probes are not suitable for this purpose. We have developed a ratiometric fluorescent probe (QG-1) for quantitatively monitoring cellular GSH. The probe responds specifically and reversibility to GSH with an ideal dissociation constant (K_d) of 2.59 mm and a fast response time (t_1/2=5.82 s). We also demonstrate that QG-1 detection of GSH is feasible in a model protein system. QG-1 was found to have extremely low cytotoxicity and was applied to determine the GSH concentration in live HeLa cells (5.40±0.87 mm).

Original language	English
Pages (from-to)	5812-5816
Number of pages	5
Journal	Angewandte Chemie - International Edition
Volume	56
Issue number	21
DOIs	https://doi.org/10.1002/anie.201702114
State	Published - 15 May 2017

Bibliographical note

Publisher Copyright:
© 2017 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim

Keywords

antioxidants
fluorescent probes
gluthionine
ratiometric probes
redox homeostasis

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10.1002/anie.201702114

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abstract = "The ability to monitor and quantify glutathione (GSH) in live cells is essential in order to gain a detailed understanding of GSH-related pathological events. However, owing to their irreversible response mechanisms, most existing fluorescent GSH probes are not suitable for this purpose. We have developed a ratiometric fluorescent probe (QG-1) for quantitatively monitoring cellular GSH. The probe responds specifically and reversibility to GSH with an ideal dissociation constant (Kd) of 2.59 mm and a fast response time (t1/2=5.82 s). We also demonstrate that QG-1 detection of GSH is feasible in a model protein system. QG-1 was found to have extremely low cytotoxicity and was applied to determine the GSH concentration in live HeLa cells (5.40±0.87 mm).",

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AU - Wu, Xue

AU - Yoon, Juyoung

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KW - gluthionine

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A Reversible Fluorescent Probe for Real-Time Quantitative Monitoring of Cellular Glutathione

Abstract

Bibliographical note

Keywords

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