A Reversible Fluorescent Probe for Real-Time Quantitative Monitoring of Cellular Glutathione

Zhixue Liu, Xin Zhou, Yu Miao, Ying Hu, Nahyun Kwon, Xue Wu, Juyoung Yoon

Research output: Contribution to journalArticlepeer-review

190 Scopus citations

Abstract

The ability to monitor and quantify glutathione (GSH) in live cells is essential in order to gain a detailed understanding of GSH-related pathological events. However, owing to their irreversible response mechanisms, most existing fluorescent GSH probes are not suitable for this purpose. We have developed a ratiometric fluorescent probe (QG-1) for quantitatively monitoring cellular GSH. The probe responds specifically and reversibility to GSH with an ideal dissociation constant (Kd) of 2.59 mm and a fast response time (t1/2=5.82 s). We also demonstrate that QG-1 detection of GSH is feasible in a model protein system. QG-1 was found to have extremely low cytotoxicity and was applied to determine the GSH concentration in live HeLa cells (5.40±0.87 mm).

Original languageEnglish
Pages (from-to)5812-5816
Number of pages5
JournalAngewandte Chemie - International Edition
Volume56
Issue number21
DOIs
StatePublished - 15 May 2017

Keywords

  • antioxidants
  • fluorescent probes
  • gluthionine
  • ratiometric probes
  • redox homeostasis

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