A resettable high-density microfluidic cell trapping system

Ryan D. Sochol, Kosuke Iwai, Adrienne T. Higa, Joanne C. Lo, Eric Zhou, Liana Lo, Chris Luong, Megan Dueck, Song Li, Luke P. Lee, Liwei Lin

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Scopus citations

Abstract

Dynamic cell-based microarrays offer numerous benefits for biological applications such as cytokine detection, medical diagnostics, drug screening, and cellomics; however, current cell arraying techniques remain limited in terms of trapping density and device resettability. Here we demonstrate a resettable cell arraying system by utilizing simple geometric designs in microfluidic devices to manipulate microscale hydrodynamics. We show that arrays of individual bovine aortic endothelial cells (BAECs): (i) immobilize in designated trapping positions between microposts under forward fluidic flow, and (ii) release from the trapping sites under backward fluidic flow of 0.5X trypsin to reset the arrays.

Original languageEnglish
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages1574-1576
Number of pages3
StatePublished - 2010
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: 3 Oct 20107 Oct 2010

Publication series

Name14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Volume3

Conference

Conference14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Country/TerritoryNetherlands
CityGroningen
Period3/10/107/10/10

Keywords

  • Cell arraying
  • Cell trapping
  • Cell-based microarrays
  • Microparticle arraying

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