A ratiometric fluorescent probe based on a coumarin-hemicyanine scaffold for sensitive and selective detection of endogenous peroxynitrite

Xin Zhou, Younghee Kwon, Gyoungmi Kim, Ji Hwan Ryu, Juyoung Yoon

Research output: Contribution to journalArticlepeer-review

138 Scopus citations

Abstract

In the study described herein, the red emitting probe CHCN, which possesses a linked coumarin-hemicyanine scaffold, was developed for detection of peroxynitrite (ONOO-) under physiological conditions. The studies show that CHCN displays a dual ratiometric and colorimetric response to ONOO- that is caused by an oxidation process. A possible mechanism of this oxidation process was proposed and confirmed by ESI-MS spectra for the first time. CHCN shown highly selective and sensitive towards ONOO- with a low limit of detection LOD (49.7nM). Moreover, CHCN has appreciable cell permeability and, as a result, it is applicable to ratiometric detection of exogenous and endogenous ONOO- in living cells during phagocytic immune response. We anticipate that, owing to their ideal properties, probes of this type will find great use in explorations of the role played by ONOO- in biology.

Original languageEnglish
Pages (from-to)285-291
Number of pages7
JournalBiosensors and Bioelectronics
Volume64
DOIs
StatePublished - 15 Feb 2015

Keywords

  • Cell imaging
  • Fluorescent chemosensor
  • ONOO sensor
  • Peroxynitrite sensor
  • Reactive oxygen species probe

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