A ratiometric fluorescent probe based on a coumarin-hemicyanine scaffold for sensitive and selective detection of endogenous peroxynitrite

Xin Zhou, Younghee Kwon, Gyoungmi Kim, Ji Hwan Ryu, Juyoung Yoon

Research output: Contribution to journalArticlepeer-review

153 Scopus citations

Abstract

In the study described herein, the red emitting probe CHCN, which possesses a linked coumarin-hemicyanine scaffold, was developed for detection of peroxynitrite (ONOO-) under physiological conditions. The studies show that CHCN displays a dual ratiometric and colorimetric response to ONOO- that is caused by an oxidation process. A possible mechanism of this oxidation process was proposed and confirmed by ESI-MS spectra for the first time. CHCN shown highly selective and sensitive towards ONOO- with a low limit of detection LOD (49.7nM). Moreover, CHCN has appreciable cell permeability and, as a result, it is applicable to ratiometric detection of exogenous and endogenous ONOO- in living cells during phagocytic immune response. We anticipate that, owing to their ideal properties, probes of this type will find great use in explorations of the role played by ONOO- in biology.

Original languageEnglish
Pages (from-to)285-291
Number of pages7
JournalBiosensors and Bioelectronics
Volume64
DOIs
StatePublished - 15 Feb 2015

Bibliographical note

Funding Information:
This research was supported by a grant from the National Creative Research Initiative programs of the National Research Foundation of Korea (NRF) funded by the Korean Government ( Ministry of Science, ICT and Future Planning ) (No. 2012R1A3A2048814 ). J.-H.R. thanks for the Basic Science Research Program through the NRF funded by the Ministry of Education (Grant 2013R1A1A2008511 ), and the Bio & Medical Technology Development Program of the NRF funded by Ministry of Science, ICT and Future Planning ( NRF-2013M3A9D5072551 ).

Publisher Copyright:
© 2014 Elsevier B.V.

Keywords

  • Cell imaging
  • Fluorescent chemosensor
  • ONOO sensor
  • Peroxynitrite sensor
  • Reactive oxygen species probe

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