TY - JOUR
T1 - A ratiometric fluorescent probe based on a coumarin-hemicyanine scaffold for sensitive and selective detection of endogenous peroxynitrite
AU - Zhou, Xin
AU - Kwon, Younghee
AU - Kim, Gyoungmi
AU - Ryu, Ji Hwan
AU - Yoon, Juyoung
N1 - Funding Information:
This research was supported by a grant from the National Creative Research Initiative programs of the National Research Foundation of Korea (NRF) funded by the Korean Government ( Ministry of Science, ICT and Future Planning ) (No. 2012R1A3A2048814 ). J.-H.R. thanks for the Basic Science Research Program through the NRF funded by the Ministry of Education (Grant 2013R1A1A2008511 ), and the Bio & Medical Technology Development Program of the NRF funded by Ministry of Science, ICT and Future Planning ( NRF-2013M3A9D5072551 ).
Publisher Copyright:
© 2014 Elsevier B.V.
PY - 2015/2/15
Y1 - 2015/2/15
N2 - In the study described herein, the red emitting probe CHCN, which possesses a linked coumarin-hemicyanine scaffold, was developed for detection of peroxynitrite (ONOO-) under physiological conditions. The studies show that CHCN displays a dual ratiometric and colorimetric response to ONOO- that is caused by an oxidation process. A possible mechanism of this oxidation process was proposed and confirmed by ESI-MS spectra for the first time. CHCN shown highly selective and sensitive towards ONOO- with a low limit of detection LOD (49.7nM). Moreover, CHCN has appreciable cell permeability and, as a result, it is applicable to ratiometric detection of exogenous and endogenous ONOO- in living cells during phagocytic immune response. We anticipate that, owing to their ideal properties, probes of this type will find great use in explorations of the role played by ONOO- in biology.
AB - In the study described herein, the red emitting probe CHCN, which possesses a linked coumarin-hemicyanine scaffold, was developed for detection of peroxynitrite (ONOO-) under physiological conditions. The studies show that CHCN displays a dual ratiometric and colorimetric response to ONOO- that is caused by an oxidation process. A possible mechanism of this oxidation process was proposed and confirmed by ESI-MS spectra for the first time. CHCN shown highly selective and sensitive towards ONOO- with a low limit of detection LOD (49.7nM). Moreover, CHCN has appreciable cell permeability and, as a result, it is applicable to ratiometric detection of exogenous and endogenous ONOO- in living cells during phagocytic immune response. We anticipate that, owing to their ideal properties, probes of this type will find great use in explorations of the role played by ONOO- in biology.
KW - Cell imaging
KW - Fluorescent chemosensor
KW - ONOO sensor
KW - Peroxynitrite sensor
KW - Reactive oxygen species probe
UR - http://www.scopus.com/inward/record.url?scp=84907197316&partnerID=8YFLogxK
U2 - 10.1016/j.bios.2014.08.089
DO - 10.1016/j.bios.2014.08.089
M3 - Article
C2 - 25240128
AN - SCOPUS:84907197316
SN - 0956-5663
VL - 64
SP - 285
EP - 291
JO - Biosensors and Bioelectronics
JF - Biosensors and Bioelectronics
ER -