A pyrene-imidazolium derivative that selectively Recognizes G-Quadruplex DNA

Ha Na Kim, Eun Hae Lee, Zhaochao Xu, Hee Eun Kim, Hee Seung Lee, Joon Hwa Lee, Juyoung Yoon

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

G-quadruplexes, formed of four stranded guanine bases stabilized by monovalent cations, serve important role in cancer cell growth and control gene expression in telomere. Since there are various types of quadruplex structures, rapid and simple screening methods with high selectivity, sensitivity and nontoxicity are required for understanding about the biological roles of quadruplex DNA as well as in designing therapeutics. Herein, we report a pyrene-imidazolium derivative, JY-1, which can with high selectivity recognize G-quadruplex using fluorescence and NMR spectroscopy. This is the first example based on the imidazolium derivative, which can detect the G-quadruplex directly to utilize the excimer/monomer emission change in pyrene fluorophore. The selectivity of strong binding to a specific sequence can allow for quadruplex sensing and the detection method presented here is very simple, using fluorescence and NMR study. Also, the groove binding characteristic of JY-1 to the G-quadruplex has a relatively low nonspecific toxicity and the structure-specific differences in fluorescent character between DNA duplex and G-quadruplex may offer more discovery and application in biological study.

Original languageEnglish
Pages (from-to)2282-2288
Number of pages7
JournalBiomaterials
Volume33
Issue number7
DOIs
StatePublished - Mar 2012

Keywords

  • Excimer emission
  • Fluorescent sensor
  • G-quadruplex
  • Imidazolium
  • NMR
  • Pyrene

Fingerprint

Dive into the research topics of 'A pyrene-imidazolium derivative that selectively Recognizes G-Quadruplex DNA'. Together they form a unique fingerprint.

Cite this