A protein transduction domain located at the NH2-terminus of human translationally controlled tumor protein for delivery of active molecules to cells

Moonhee Kim, Miyoung Kim, Hyo Young Kim, Sol Kim, Jaehoon Jung, Jeehye Maeng, Jun Chang, Kyunglim Lee

Research output: Contribution to journalArticlepeer-review

27 Scopus citations

Abstract

Protein transduction domains (PTDs) are small peptides, able to penetrate biological membranes and deliver various types of cargo both in vitro and in vivo. Because use of PTDs originating from viral origins resulted in undesired effects, PTDs originating from non-viral origins are needed. Here, we report that a 10-amino acid peptide (MIIYRDLISH) derived from the NH2-terminus of human translationally controlled tumor protein (TCTP) functions as a PTD. This peptide was internalized through lipid raft-dependent endocytosis and partial macropinocytosis, and did not enter lysosome and nucleus. Beta-galactosidase fused to TCTP-PTD, when injected into mice, was efficiently delivered to liver, kidney, spleen, heart, and lungs of the animals. Preincubation of TCTP-PTD with adenovirus increased adenoviral mediated-gene expression in cells and also improved immune response to intranasally administered adenovirus expressing the triple repeat of G glycoprotein of respiratory syncytial virus (RSV), rAd/3×G. These findings suggest that TCTP-PTD might overcome the limitations of polycation-mediated transduction and serve as an efficient vehicle for drug delivery.

Original languageEnglish
Pages (from-to)222-230
Number of pages9
JournalBiomaterials
Volume32
Issue number1
DOIs
StatePublished - Jan 2011

Keywords

  • Adenovirus
  • Endocytosis
  • Protein transduction domain
  • TCTP

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