TY - JOUR
T1 - A preliminary X-ray study of transketolase from Burkholderia pseudomallei
AU - Kim, Mi Sun
AU - Lim, Areum
AU - Yang, Seung Won
AU - Lee, Daeun
AU - Park, Jimin
AU - Shin, Dong Hae
PY - 2012/12
Y1 - 2012/12
N2 - ATktA is the most critical enzyme in the nonoxidative pentose phosphate pathway. It catalyzes the conversion of xylulose 5-phosphate and ribose 5 - phosphate into sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate, and its products are used in the biosynthesis of acetyl-CoA, aromatic amino acids, nucleic acids and ADP-l-glycero-β-d-manno-heptose. TktA also has an unexpected role in chromosome structure that is independent of its metabolic responsibilities. Therefore, it is a new potent antibiotic target. In this study, TktA from Burkholderia pseudomallei has been cloned, expressed, purified and crystallized. Synchrotron X-ray data were also collected to 2.0 A resolution. The crystal belonged to the monoclinic space group C2, with unit-cell parameters a = 146.2, b = 74.6, c = 61.6 A, β = 113.0°. A full structural determination is under way in order to provide insight into the structure-function relationship of this protein.
AB - ATktA is the most critical enzyme in the nonoxidative pentose phosphate pathway. It catalyzes the conversion of xylulose 5-phosphate and ribose 5 - phosphate into sedoheptulose 7-phosphate and glyceraldehyde 3-phosphate, and its products are used in the biosynthesis of acetyl-CoA, aromatic amino acids, nucleic acids and ADP-l-glycero-β-d-manno-heptose. TktA also has an unexpected role in chromosome structure that is independent of its metabolic responsibilities. Therefore, it is a new potent antibiotic target. In this study, TktA from Burkholderia pseudomallei has been cloned, expressed, purified and crystallized. Synchrotron X-ray data were also collected to 2.0 A resolution. The crystal belonged to the monoclinic space group C2, with unit-cell parameters a = 146.2, b = 74.6, c = 61.6 A, β = 113.0°. A full structural determination is under way in order to provide insight into the structure-function relationship of this protein.
KW - Burkholderia pseudomallei
KW - Tkt
KW - transketolase
UR - http://www.scopus.com/inward/record.url?scp=84870925001&partnerID=8YFLogxK
U2 - 10.1107/S1744309112044375
DO - 10.1107/S1744309112044375
M3 - Article
C2 - 23192046
AN - SCOPUS:84870925001
SN - 1744-3091
VL - 68
SP - 1554
EP - 1556
JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
IS - 12
ER -