TY - JOUR
T1 - A preliminary X-ray study of sedoheptulose-7-phosphate isomerase from Burkholderia pseudomallei
AU - Kim, Mi Sun
AU - Shin, Dong Hae
PY - 2009
Y1 - 2009
N2 - Sedoheptulose-7-phosphate isomerase (GmhA) converts d-sedoheptulose 7 - phosphate to d,d-heptose 7-phosphate. This is the first step in the biosynthesis pathway of NDP-heptose, which is responsible for the pleiotropic phenotype. This biosynthesis pathway is the target of inhibitors to increase the membrane permeability of Gram-negative pathogens or of adjuvants working synergistically with known antibiotics. Burkholderia pseudomallei is the causative agent of melioidosis, a seriously invasive disease in animals and humans in tropical and subtropical areas. GmhA from B. pseudomallei is one of the targets of antibiotic adjuvants for melioidosis. In this study, GmhA has been cloned, expressed, purified and crystallized. Synchrotron X-ray data were also collected to 1.9 Å resolution. The crystal belonged to the primitive orthorhombic space group P212121, with unit-cell parameters a = 61.3, b = 84.2, c = 142.3 Å. A full structural determination is under way in order to provide insights into the structure-function relationships of this protein.
AB - Sedoheptulose-7-phosphate isomerase (GmhA) converts d-sedoheptulose 7 - phosphate to d,d-heptose 7-phosphate. This is the first step in the biosynthesis pathway of NDP-heptose, which is responsible for the pleiotropic phenotype. This biosynthesis pathway is the target of inhibitors to increase the membrane permeability of Gram-negative pathogens or of adjuvants working synergistically with known antibiotics. Burkholderia pseudomallei is the causative agent of melioidosis, a seriously invasive disease in animals and humans in tropical and subtropical areas. GmhA from B. pseudomallei is one of the targets of antibiotic adjuvants for melioidosis. In this study, GmhA has been cloned, expressed, purified and crystallized. Synchrotron X-ray data were also collected to 1.9 Å resolution. The crystal belonged to the primitive orthorhombic space group P212121, with unit-cell parameters a = 61.3, b = 84.2, c = 142.3 Å. A full structural determination is under way in order to provide insights into the structure-function relationships of this protein.
KW - Burkholderia pseudomallei
KW - Sedoheptulose-7-phosphate isomerase
UR - http://www.scopus.com/inward/record.url?scp=73449147977&partnerID=8YFLogxK
U2 - 10.1107/S174430910903259X
DO - 10.1107/S174430910903259X
M3 - Article
C2 - 19923728
AN - SCOPUS:73449147977
SN - 1744-3091
VL - 65
SP - 1110
EP - 1112
JO - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
JF - Acta Crystallographica Section F: Structural Biology and Crystallization Communications
IS - 11
ER -