A preliminary X-ray study of murine Tnfaip8/Oxi-α

Daeun Lee; Mi Sun Kim; Jimin Park; Gil Ja Jhon; Jin H. Son; Dong Hae Shin

doi:10.3390/ijms15034523

A preliminary X-ray study of murine Tnfaip8/Oxi-α

Daeun Lee, Mi Sun Kim, Jimin Park, Gil Ja Jhon, Jin H. Son, Dong Hae Shin

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Tnfaip8/oxidative stress regulated gene-α (Oxi-α) is a novel protein expressed specifically in brain dopaminergic neurons and its over-expression has been reported to protect dopaminergic cells against OS-induced cell death. In this study, murine C165S mutant Tnfaip8/Oxi-α has been crystallized and X-ray data have been collected to 1.8 Å using synchrotron radiation. The crystal belonged to the primitive orthorhombic space group P2₁2₁2, with unit-cell parameters a = 66.9, b = 72.3, c = 93.5 Å. A full structural determination is under way in order to provide insights into the structure-function relationships of this protein.

Original language	English
Pages (from-to)	4523-4530
Number of pages	8
Journal	International Journal of Molecular Sciences
Volume	15
Issue number	3
DOIs	https://doi.org/10.3390/ijms15034523
State	Published - 14 Mar 2014

Keywords

C165S mutant
Oxi-α
Oxidative stress
Parkinson's disease
X-ray crystallography

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10.3390/ijms15034523

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title = "A preliminary X-ray study of murine Tnfaip8/Oxi-α",

abstract = "Tnfaip8/oxidative stress regulated gene-α (Oxi-α) is a novel protein expressed specifically in brain dopaminergic neurons and its over-expression has been reported to protect dopaminergic cells against OS-induced cell death. In this study, murine C165S mutant Tnfaip8/Oxi-α has been crystallized and X-ray data have been collected to 1.8 {\AA} using synchrotron radiation. The crystal belonged to the primitive orthorhombic space group P21212, with unit-cell parameters a = 66.9, b = 72.3, c = 93.5 {\AA}. A full structural determination is under way in order to provide insights into the structure-function relationships of this protein.",

keywords = "C165S mutant, Oxi-α, Oxidative stress, Parkinson's disease, X-ray crystallography",

author = "Daeun Lee and Kim, \{Mi Sun\} and Jimin Park and Jhon, \{Gil Ja\} and Son, \{Jin H.\} and Shin, \{Dong Hae\}",

year = "2014",

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doi = "10.3390/ijms15034523",

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T1 - A preliminary X-ray study of murine Tnfaip8/Oxi-α

AU - Lee, Daeun

AU - Kim, Mi Sun

AU - Park, Jimin

AU - Jhon, Gil Ja

AU - Son, Jin H.

AU - Shin, Dong Hae

PY - 2014/3/14

Y1 - 2014/3/14

N2 - Tnfaip8/oxidative stress regulated gene-α (Oxi-α) is a novel protein expressed specifically in brain dopaminergic neurons and its over-expression has been reported to protect dopaminergic cells against OS-induced cell death. In this study, murine C165S mutant Tnfaip8/Oxi-α has been crystallized and X-ray data have been collected to 1.8 Å using synchrotron radiation. The crystal belonged to the primitive orthorhombic space group P21212, with unit-cell parameters a = 66.9, b = 72.3, c = 93.5 Å. A full structural determination is under way in order to provide insights into the structure-function relationships of this protein.

AB - Tnfaip8/oxidative stress regulated gene-α (Oxi-α) is a novel protein expressed specifically in brain dopaminergic neurons and its over-expression has been reported to protect dopaminergic cells against OS-induced cell death. In this study, murine C165S mutant Tnfaip8/Oxi-α has been crystallized and X-ray data have been collected to 1.8 Å using synchrotron radiation. The crystal belonged to the primitive orthorhombic space group P21212, with unit-cell parameters a = 66.9, b = 72.3, c = 93.5 Å. A full structural determination is under way in order to provide insights into the structure-function relationships of this protein.

KW - C165S mutant

KW - Oxi-α

KW - Oxidative stress

KW - Parkinson's disease

KW - X-ray crystallography

UR - https://www.scopus.com/pages/publications/84896461774

U2 - 10.3390/ijms15034523

DO - 10.3390/ijms15034523

M3 - Article

C2 - 24637935

AN - SCOPUS:84896461774

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EP - 4530

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

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A preliminary X-ray study of murine Tnfaip8/Oxi-α

Abstract

Keywords

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