TY - JOUR
T1 - A preliminary X-ray study of 3-deoxy-d-manno-oct-2-ulosonic acid 8-phosphate phosphatase (YrbI) from Burkholderia pseudomallei
AU - Park, Jimin
AU - Lee, Daeun
AU - Kim, Mi Sun
AU - Kim, Dae Yong
AU - Shin, Dong Hae
N1 - Publisher Copyright:
© 2015 International Union of Crystallography.
PY - 2015/6/1
Y1 - 2015/6/1
N2 - 3-Deoxy-d-manno-oct-2-ulosonic acid 8-phosphate phosphatase (YrbI), the third enzyme in the pathway for the biosynthesis of 3-deoxy-d-manno-oct-2-ulosonic acid (KDO), hydrolyzes KDO 8-phosphate to KDO and inorganic phosphate. YrbI belongs to the haloacid dehalogenase (HAD) superfamily, which is a large family of magnesium-dependent phosphatase/phospho transferase enzymes. In this study, YrbI from Burkholderia pseudomallei, the causative agent of melioidosis, has been cloned, expressed, purified and crystallized. Synchrotron X-ray data were also collected to 2.25 Å resolution. The crystal belonged to the primitive orthorhombic space group P212121, with unit-cell parameters a = 63.7, b = 97.5, c = 98.0 Å. A full structural determination is in progress to elucidate the structure-function relationship of this protein.
AB - 3-Deoxy-d-manno-oct-2-ulosonic acid 8-phosphate phosphatase (YrbI), the third enzyme in the pathway for the biosynthesis of 3-deoxy-d-manno-oct-2-ulosonic acid (KDO), hydrolyzes KDO 8-phosphate to KDO and inorganic phosphate. YrbI belongs to the haloacid dehalogenase (HAD) superfamily, which is a large family of magnesium-dependent phosphatase/phospho transferase enzymes. In this study, YrbI from Burkholderia pseudomallei, the causative agent of melioidosis, has been cloned, expressed, purified and crystallized. Synchrotron X-ray data were also collected to 2.25 Å resolution. The crystal belonged to the primitive orthorhombic space group P212121, with unit-cell parameters a = 63.7, b = 97.5, c = 98.0 Å. A full structural determination is in progress to elucidate the structure-function relationship of this protein.
KW - 3-deoxy-d-manno-oct-2-ulosonic acid 8-phosphate phosphatase
KW - Burkholderia pseudomallei
KW - YrbI
UR - http://www.scopus.com/inward/record.url?scp=84931097199&partnerID=8YFLogxK
U2 - 10.1107/S2053230X15006135
DO - 10.1107/S2053230X15006135
M3 - Article
C2 - 26057814
AN - SCOPUS:84931097199
SN - 2053-230X
VL - 71
SP - 790
EP - 793
JO - Acta Crystallographica Section F:Structural Biology Communications
JF - Acta Crystallographica Section F:Structural Biology Communications
ER -