A Novel Long-term, Multi-Channel and Non-invasive Electrophysiology Platform for Zebrafish

Soon Gweon Hong, Philip Lee, Scott C. Baraban, Luke P. Lee

Research output: Contribution to journalArticlepeer-review

55 Scopus citations

Abstract

Zebrafish are a popular vertebrate model for human neurological disorders and drug discovery. Although fecundity, breeding convenience, genetic homology and optical transparency have been key advantages, laborious and invasive procedures are required for electrophysiological studies. Using an electrode-integrated microfluidic system, here we demonstrate a novel multichannel electrophysiology unit to record multiple zebrafish. This platform allows spontaneous alignment of zebrafish and maintains, over days, close contact between head and multiple surface electrodes, enabling non-invasive long-term electroencephalographic recording. First, we demonstrate that electrographic seizure events, induced by pentylenetetrazole, can be reliably distinguished from eye or tail movement artifacts, and quantifiably identified with our unique algorithm. Second, we show long-term monitoring during epileptogenic progression in a scn1lab mutant recapitulating human Dravet syndrome. Third, we provide an example of cross-over pharmacology antiepileptic drug testing. Such promising features of this integrated microfluidic platform will greatly facilitate high-throughput drug screening and electrophysiological characterization of epileptic zebrafish.

Original languageEnglish
Article number28248
JournalScientific Reports
Volume6
DOIs
StatePublished - 16 Jun 2016

Bibliographical note

Funding Information:
S.C.B. acknowledges funding of NINDS R01 NS079214 and Raymond & Beverley Sackler Center.

Fingerprint

Dive into the research topics of 'A Novel Long-term, Multi-Channel and Non-invasive Electrophysiology Platform for Zebrafish'. Together they form a unique fingerprint.

Cite this