Abstract
Integrated microfluidic surface-enhanced Raman spectroscopy on a compact disk (SERS-CD) platform has been developed for high-throughput quantitative biomedicine. A smart design of a SERS-CD platform including efficient cell-trapping microfluidics, a locally integrated SERS probe, and a sample-accumulating concentrator is a solution for label-free high-throughput screening and detection. A SERS probe combined with a protein concentrator allows monitoring the dynamics of secreted proteins as a function of trapped cell density, accumulation time, and the induced centrifugal force. The design of centrifugal cell trapping microfluidics was characterized by using polystyrene beads and HeLa cells and the preliminary evaluation of a centrifugal sample concentrator on a SERS probe was accomplished by observing the increased Raman spectrum of Rhodamin 6G (R6G) as the molecule accumulated on the probe.
| Original language | English |
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| Pages | 642-644 |
| Number of pages | 3 |
| State | Published - 2006 |
| Event | 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 - Tokyo, Japan Duration: 5 Nov 2006 → 9 Nov 2006 |
Conference
| Conference | 10th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2006 |
|---|---|
| Country/Territory | Japan |
| City | Tokyo |
| Period | 5/11/06 → 9/11/06 |
Keywords
- Cell trapping
- Centrifugal concentrator
- Centrifugal separation
- Quantitative cell analysis
- SERS
- SERS-CD