Abstract
We proposed a novel 3D-2D co-culture droplet array system to analyze tumor angiogenesis in a high throughput manner. A two-layer PDMS chip with 6 ×9 paired-well array was fabricated for cell co-culture. Each paired-well was composed of a TC well for Matrigel-coated tumor cells aggregated into 3D spheroids within droplet with a hypoxia-center similar to real tumor tissues, and an EC well for HUVECs cultured in 2D within droplet. Co-cultured tumor cells were able to interact with HUVECs and induce angiogenesis. Controlled by an automated DropLab system, multiple paired samples could be analyzed simultaneously in high-throughput through the droplet array.
| Original language | English |
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| Title of host publication | MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
| Publisher | Chemical and Biological Microsystems Society |
| Pages | 565-567 |
| Number of pages | 3 |
| ISBN (Electronic) | 9780979806483 |
| State | Published - 2015 |
| Event | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of Duration: 25 Oct 2015 → 29 Oct 2015 |
Publication series
| Name | MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences |
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Conference
| Conference | 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 |
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| Country/Territory | Korea, Republic of |
| City | Gyeongju |
| Period | 25/10/15 → 29/10/15 |
Bibliographical note
Publisher Copyright:© 15CBMS-0001.
Keywords
- Co-culture
- High-throughput
- Microfluidic droplet array
- Tumor angiogenesis