TY - JOUR
T1 - A Nonconsumptive Fluorescent Probe for Precise Detection of Hydrogen Peroxide in Nonalcoholic Fatty Liver Disease and Inflammation
AU - Qin, Guixin
AU - Zhang, Hanbo
AU - Shen, Wei
AU - Wang, Yuting
AU - Yin, Nan
AU - Nie, Chenyao
AU - Yoon, Juyoung
AU - Xu, Qingling
N1 - Publisher Copyright:
© 2025 American Chemical Society.
PY - 2025/4/8
Y1 - 2025/4/8
N2 - Hydrogen peroxide (H2O2) plays a vital role in various physiological and pathological processes. Thus, fluorescent probes of H2O2 are powerful tools for the investigation of H2O2-related diseases. However, developing fluorescent probes that do not irreversibly consume H2O2 presents a significant challenge. In this work, we introduce carbonate ester as a nonconsumptive recognizing molecule to construct RES-6C as a novel fluorescent probe of H2O2. RES-6C exhibited a selective and sensitive turn-on fluorescence response to H2O2, enabling the detection of H2O2 in cells without disturbing the cellular redox status. RES-6C has been applied to study nonalcoholic fatty liver disease, revealing that peroxisomes and mitochondria contribute to H2O2 production to a similar extent during very-long-chain fatty acid metabolism for the first time. It has also enabled fluorescent imaging of H2O2 in the LPS-induced inflammation mouse model.
AB - Hydrogen peroxide (H2O2) plays a vital role in various physiological and pathological processes. Thus, fluorescent probes of H2O2 are powerful tools for the investigation of H2O2-related diseases. However, developing fluorescent probes that do not irreversibly consume H2O2 presents a significant challenge. In this work, we introduce carbonate ester as a nonconsumptive recognizing molecule to construct RES-6C as a novel fluorescent probe of H2O2. RES-6C exhibited a selective and sensitive turn-on fluorescence response to H2O2, enabling the detection of H2O2 in cells without disturbing the cellular redox status. RES-6C has been applied to study nonalcoholic fatty liver disease, revealing that peroxisomes and mitochondria contribute to H2O2 production to a similar extent during very-long-chain fatty acid metabolism for the first time. It has also enabled fluorescent imaging of H2O2 in the LPS-induced inflammation mouse model.
UR - https://www.scopus.com/pages/publications/105002589515
U2 - 10.1021/acs.analchem.4c06647
DO - 10.1021/acs.analchem.4c06647
M3 - Article
AN - SCOPUS:105002589515
SN - 0003-2700
VL - 97
SP - 7195
EP - 7202
JO - Analytical Chemistry
JF - Analytical Chemistry
IS - 13
ER -