TY - JOUR
T1 - A new kind of rhodamine-based fluorescence turn-on probe for monitoring ATP in mitochondria
AU - Liu, Yifan
AU - Lee, Dayoung
AU - Wu, Di
AU - Swamy, K. M.K.
AU - Yoon, Juyoung
N1 - Funding Information:
This research study was supported by a grant from the National Creative Research Initiative Program of the National Research Foundation of Korea (NRF), which is funded by the Korean government (MSIP) (No. 2012R1A3A2048814 ). Mass spectral data were obtained from the Korea Basic Science Institute (Daegu), by Jeol JMS 700 high-resolution mass spectrometry. Appendix A
Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2018/7/15
Y1 - 2018/7/15
N2 - We have developed a new kind of colorimetric and fluorescence “turn-on” probe based on rhodamine derivatives for the detection of adenosine-5′-triphosphate (ATP) through hydrogen bond interactions. Upon the addition of 5 mM ATP, the probe 1 shows a 65-fold enhancement in fluorescence intensity and a color change from colorless to pink. The probe 2 also shows very obvious color and fluorescence change with the presence of ATP. The experimental results show that this kind of probe has high specificity towards ATP over other anions, metal ions, and other nucleoside polyphosphates, except ADP. The enhanced emission and naked eye changes are attributed to spirolactam ring-opening, which is pH independent (pH (4.0–7.4)). The more important finding from our results is that the electron withdrawing group in the chain decreases the binding ability to ATP. The live cell imaging experiments in Hela cells indicated that probe 2 has cell permeability, and mainly locates to mitochondria.
AB - We have developed a new kind of colorimetric and fluorescence “turn-on” probe based on rhodamine derivatives for the detection of adenosine-5′-triphosphate (ATP) through hydrogen bond interactions. Upon the addition of 5 mM ATP, the probe 1 shows a 65-fold enhancement in fluorescence intensity and a color change from colorless to pink. The probe 2 also shows very obvious color and fluorescence change with the presence of ATP. The experimental results show that this kind of probe has high specificity towards ATP over other anions, metal ions, and other nucleoside polyphosphates, except ADP. The enhanced emission and naked eye changes are attributed to spirolactam ring-opening, which is pH independent (pH (4.0–7.4)). The more important finding from our results is that the electron withdrawing group in the chain decreases the binding ability to ATP. The live cell imaging experiments in Hela cells indicated that probe 2 has cell permeability, and mainly locates to mitochondria.
KW - ATP sensor
KW - Colorimetric chemosensor
KW - Fluorescence sensor
KW - Mitochondria targeting
KW - Rhodamine
UR - http://www.scopus.com/inward/record.url?scp=85044141822&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2018.03.081
DO - 10.1016/j.snb.2018.03.081
M3 - Article
AN - SCOPUS:85044141822
SN - 0925-4005
VL - 265
SP - 429
EP - 434
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
ER -