TY - JOUR
T1 - A near-infrared fluorescent probe for amyloid-β aggregates
AU - Lee, Misun
AU - Kim, Mingeun
AU - Tikum, Anjong Florence
AU - Lee, Hyuck Jin
AU - Thamilarasan, Vijayan
AU - Lim, Mi Hee
AU - Kim, Jinheung
N1 - Funding Information:
This work is supported by “Next Generation Carbon Upcycling Project” [Project No. 2017M1A2A2042517 (to J.K.)] through the National Research Foundation (NRF) funded by the Ministry of Science and ICT and the NRF grant funded by the Korean government [NRF-2017R1A5A1015365 (to J.K.); NRF-2016R1A5A1009405 and NRF-2017R1A2B3002585 (to M.H.L.)].
Funding Information:
This work is supported by “Next Generation Carbon Upcycling Project” [Project No. 2017M1A2A2042517 (to J.K.)] through the National Research Foundation (NRF) funded by the Ministry of Science and ICT and the NRF grant funded by the Korean government [NRF- 2017R1A5A1015365 (to J.K.); NRF-2016R1A5A1009405 and NRF-2017R1A2B3002585 (to M.H.L.)].
Publisher Copyright:
© 2018 Elsevier Ltd
PY - 2019/3
Y1 - 2019/3
N2 - The deposition of amyloid-β (Aβ) aggregates in the brain is a hallmark of the Alzheimer's disease (AD)-affected brain. Various aggregated Aβ species, including oligomers and fibrils, are generated upon the aggregation process, and these Aβ aggregates have distinct biological properties. To non-invasively monitor the aggregation pathways of Aβ and identify the existence of certain Aβ species in biological environments, an effective strategy to detect Aβ species is necessary. Herein, we report a turn-on near-infrared (near-IR) fluorescent probe, 1, for Aβ aggregates, which consists of a donor-π-acceptor system with an Aβ-interacting moiety. Our probe, 1, shows a noticeable increase in near-IR fluorescence at ca. 710 nm in the presence of Aβ aggregates in aqueous media. In addition, the fluorescent response of 1 was altered depending on the degree of Aβ aggregation. Moreover, 1 indicates turn-on fluorescence with Aβ aggregates in living cells and is nontoxic under the condition used for live-cell imaging.
AB - The deposition of amyloid-β (Aβ) aggregates in the brain is a hallmark of the Alzheimer's disease (AD)-affected brain. Various aggregated Aβ species, including oligomers and fibrils, are generated upon the aggregation process, and these Aβ aggregates have distinct biological properties. To non-invasively monitor the aggregation pathways of Aβ and identify the existence of certain Aβ species in biological environments, an effective strategy to detect Aβ species is necessary. Herein, we report a turn-on near-infrared (near-IR) fluorescent probe, 1, for Aβ aggregates, which consists of a donor-π-acceptor system with an Aβ-interacting moiety. Our probe, 1, shows a noticeable increase in near-IR fluorescence at ca. 710 nm in the presence of Aβ aggregates in aqueous media. In addition, the fluorescent response of 1 was altered depending on the degree of Aβ aggregation. Moreover, 1 indicates turn-on fluorescence with Aβ aggregates in living cells and is nontoxic under the condition used for live-cell imaging.
KW - Amyloid-β
KW - Benzo[e]indole
KW - Dimethylaminostyrene
KW - Donor-π-acceptor system
KW - Fluorescent probe
UR - http://www.scopus.com/inward/record.url?scp=85054860215&partnerID=8YFLogxK
U2 - 10.1016/j.dyepig.2018.10.013
DO - 10.1016/j.dyepig.2018.10.013
M3 - Article
AN - SCOPUS:85054860215
SN - 0143-7208
VL - 162
SP - 97
EP - 103
JO - Dyes and Pigments
JF - Dyes and Pigments
ER -