A near-infrared fluorescent probe for amyloid-β aggregates

Misun Lee, Mingeun Kim, Anjong Florence Tikum, Hyuck Jin Lee, Vijayan Thamilarasan, Mi Hee Lim, Jinheung Kim

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

The deposition of amyloid-β (Aβ) aggregates in the brain is a hallmark of the Alzheimer's disease (AD)-affected brain. Various aggregated Aβ species, including oligomers and fibrils, are generated upon the aggregation process, and these Aβ aggregates have distinct biological properties. To non-invasively monitor the aggregation pathways of Aβ and identify the existence of certain Aβ species in biological environments, an effective strategy to detect Aβ species is necessary. Herein, we report a turn-on near-infrared (near-IR) fluorescent probe, 1, for Aβ aggregates, which consists of a donor-π-acceptor system with an Aβ-interacting moiety. Our probe, 1, shows a noticeable increase in near-IR fluorescence at ca. 710 nm in the presence of Aβ aggregates in aqueous media. In addition, the fluorescent response of 1 was altered depending on the degree of Aβ aggregation. Moreover, 1 indicates turn-on fluorescence with Aβ aggregates in living cells and is nontoxic under the condition used for live-cell imaging.

Original languageEnglish
Pages (from-to)97-103
Number of pages7
JournalDyes and Pigments
Volume162
DOIs
StatePublished - Mar 2019

Bibliographical note

Funding Information:
This work is supported by “Next Generation Carbon Upcycling Project” [Project No. 2017M1A2A2042517 (to J.K.)] through the National Research Foundation (NRF) funded by the Ministry of Science and ICT and the NRF grant funded by the Korean government [NRF-2017R1A5A1015365 (to J.K.); NRF-2016R1A5A1009405 and NRF-2017R1A2B3002585 (to M.H.L.)].

Funding Information:
This work is supported by “Next Generation Carbon Upcycling Project” [Project No. 2017M1A2A2042517 (to J.K.)] through the National Research Foundation (NRF) funded by the Ministry of Science and ICT and the NRF grant funded by the Korean government [NRF- 2017R1A5A1015365 (to J.K.); NRF-2016R1A5A1009405 and NRF-2017R1A2B3002585 (to M.H.L.)].

Publisher Copyright:
© 2018 Elsevier Ltd

Keywords

  • Amyloid-β
  • Benzo[e]indole
  • Dimethylaminostyrene
  • Donor-π-acceptor system
  • Fluorescent probe

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