A microfluidic platform optimizing bead-based ELISA for the detection of cell secretion

B. Lincoln; J. Garcia Cordero; C. R. Poulsen; L. P. Lee

A microfluidic platform optimizing bead-based ELISA for the detection of cell secretion

B. Lincoln, J. Garcia Cordero, C. R. Poulsen, L. P. Lee

Department of Chemistry & Nanoscience

Research output: Contribution to conference › Paper › peer-review

Abstract

With the goal of enabling the dynamic measurement of cell secretion within a micro-fluidic cell culturing platform, we have developed chambers that efficiently deliver the surrounding media to a bead-based ELISA detection site while maintaining optimal culture conditions. Platforms for the optimization of this system are introduced, including a size-varied chamber array that can test for the number of cells required for a detectable signal.

Original language	English
Pages	1810-1812
Number of pages	3
State	Published - 2008
Event	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States Duration: 12 Oct 2008 → 16 Oct 2008

Conference

Conference	12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
Country/Territory	United States
City	San Diego, CA
Period	12/10/08 → 16/10/08

Keywords

Culture platform
ELISA
Secretion
Systems biology

Cite this

@conference{4943936a67ac47e7a63d91de8efe6b0f,

title = "A microfluidic platform optimizing bead-based ELISA for the detection of cell secretion",

abstract = "With the goal of enabling the dynamic measurement of cell secretion within a micro-fluidic cell culturing platform, we have developed chambers that efficiently deliver the surrounding media to a bead-based ELISA detection site while maintaining optimal culture conditions. Platforms for the optimization of this system are introduced, including a size-varied chamber array that can test for the number of cells required for a detectable signal.",

keywords = "Culture platform, ELISA, Secretion, Systems biology",

author = "B. Lincoln and Cordero, {J. Garcia} and Poulsen, {C. R.} and Lee, {L. P.}",

year = "2008",

language = "English",

pages = "1810--1812",

note = "12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 ; Conference date: 12-10-2008 Through 16-10-2008",

}

A microfluidic platform optimizing bead-based ELISA for the detection of cell secretion. / Lincoln, B.; Cordero, J. Garcia; Poulsen, C. R. et al.
2008. 1810-1812 Paper presented at 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008, San Diego, CA, United States.

Research output: Contribution to conference › Paper › peer-review

TY - CONF

T1 - A microfluidic platform optimizing bead-based ELISA for the detection of cell secretion

AU - Lincoln, B.

AU - Cordero, J. Garcia

AU - Poulsen, C. R.

AU - Lee, L. P.

PY - 2008

Y1 - 2008

N2 - With the goal of enabling the dynamic measurement of cell secretion within a micro-fluidic cell culturing platform, we have developed chambers that efficiently deliver the surrounding media to a bead-based ELISA detection site while maintaining optimal culture conditions. Platforms for the optimization of this system are introduced, including a size-varied chamber array that can test for the number of cells required for a detectable signal.

AB - With the goal of enabling the dynamic measurement of cell secretion within a micro-fluidic cell culturing platform, we have developed chambers that efficiently deliver the surrounding media to a bead-based ELISA detection site while maintaining optimal culture conditions. Platforms for the optimization of this system are introduced, including a size-varied chamber array that can test for the number of cells required for a detectable signal.

KW - Culture platform

KW - ELISA

KW - Secretion

KW - Systems biology

UR - http://www.scopus.com/inward/record.url?scp=84902504215&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:84902504215

SP - 1810

EP - 1812

T2 - 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008

Y2 - 12 October 2008 through 16 October 2008

ER -

A microfluidic platform optimizing bead-based ELISA for the detection of cell secretion

Abstract

Conference

Keywords

Fingerprint

Cite this