A highly selective fluorescent probe for thiol bioimaging

Jean Bouffard, Youngmi Kim, Timothy M. Swager, Ralph Weissleder, Scott A. Hilderbrand

Research output: Contribution to journalArticlepeer-review

260 Scopus citations


A new fluorescent turn-on probe (3) for the selective sensing and bioimaging of thiols is reported. In aqueous buffer solutions at physiological pH, thiols cleave the 2,4-dinitrobenzenesulfonyl group to release the red-emissive donor-acceptor fluorophore (4). The probe displays excellent immunity to interference from nitrogen and oxygen nucleophiles and the imaging of thiols in living cells is demonstrated.

Original languageEnglish
Pages (from-to)37-40
Number of pages4
JournalOrganic Letters
Issue number1
StatePublished - 3 Jan 2008


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