A fully integrated cell-based cytotoxicity, gene and protein expression analysis platfrom

Gregor Kijanka, Ivan K. Dimov, Luke P. Lee, Jens Ducrée

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Scopus citations

Abstract

We demonstrate a novel, user-friendly biological microprocessor for highly efficient cell capture, culture, treatment and analysis. The device provides a multipurpose platform for a wide range of molecular and cell biology methods. As pilot application, we implement a cell-based cytotoxicity assay as well as gene and protein expression studies in both adherent and non-adherent mammalian cells. The gene expression studies are performed on mRNA level based on real-time Nucleic- Acid-Sequence Based Amplification (NASBA) while the protein expression analysis uses fluorescent immunostaining.

Original languageEnglish
Title of host publicationProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages691-693
Number of pages3
ISBN (Print)9780979806421
StatePublished - 2009
Event13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009 - Jeju, Korea, Republic of
Duration: 1 Nov 20095 Nov 2009

Publication series

NameProceedings of Conference, MicroTAS 2009 - The 13th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference13th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2009
Country/TerritoryKorea, Republic of
CityJeju
Period1/11/095/11/09

Keywords

  • Cell-based assays
  • Cytotoxicity
  • Gravity
  • Immunostaning
  • NASBA

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