A Far-Red-Emitting Fluorescence Probe for Sensitive and Selective Detection of Peroxynitrite in Live Cells and Tissues

Di Wu, Jae Chan Ryu, Youn Wook Chung, Dayoung Lee, Ji Hwan Ryu, Joo Heon Yoon, Juyoung Yoon

Research output: Contribution to journalArticlepeer-review

86 Scopus citations

Abstract

In this study, the far-red-emitting fluorescence probe 1, containing a rhodamine derivative and a hydrazide reactive group, was developed for peroxynitrite detection and imaging. This probe, which is cell permeable and shows high sensitivity and selectivity in fluorometric detection of peroxynitrite over other ROS/RNS, was successfully utilized to detect exogenous and endogenous peroxynitrite in HeLa and RAW 264.7 cells, respectively. More importantly, 1 can also be used to detect endogenous peroxynitrite generated in Pseudomonas aeruginosa (PAO1)-infected mouse bone marrow-derived neutrophils. We anticipate that the new probe will serve as a powerful molecular imaging tool in investigations of the role(s) played by peroxynitrite in a variety of physiological and pathological contexts. (Figure Presented).

Original languageEnglish
Pages (from-to)10924-10931
Number of pages8
JournalAnalytical Chemistry
Volume89
Issue number20
DOIs
StatePublished - 17 Oct 2017

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