A Dual Colorimetric/Fluorescence System for Determining pH Based on the Nucleophilic Addition Reaction of an o-Hydroxymerocyanine Dye

Yongkang Yue, Fangjun Huo, Songyi Lee, Caixia Yin, Juyoung Yoon, Jianbin Chao, Yongbin Zhang, Fangqin Cheng

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Owing to their ability to monitor pH in a precise and rapid manner, optical probes have widely been developed for biological and nonbiological applications. The strategies thus far employed to determine pH rely on two types of processes including reversible protonation of amine nitrogen atoms and deprotonation of phenols. We have developed a novel dual, colorimetric/fluorescence system for determining the pH of a solution. This system utilizes an o-hydroxymerocyanine dye that undergoes a nucleophilic addition reaction that subsequently causes reversible structural changes interconverting a merocyanine to a spirocyanine and a spirocyanine to a spiropyran. It was demonstrated that the dye can be employed to measure the pH of solutions in the 2.5-5.75 and 9.6-11.8 ranges with color changes from yellow to dark blue and then to lavender. Moreover, the fluorescence response associated with the spirocyanine-spiropyran transformation of the dye occurring in alkaline solutions provides a precise method. Strike that, reverse it! A novel dual, colorimetric/fluorescence system for determining the pH of a solution has been developed. This system utilizes an o-hydroxymerocyanine dye, which undergoes a nucleophilic addition reaction that subsequently causes reversible structural changes interconverting a merocyanine to a spirocyanine and a spirocyanine to a spiropyran (see scheme).

Original languageEnglish
Pages (from-to)1239-1243
Number of pages5
JournalChemistry - A European Journal
Volume22
Issue number4
DOIs
StatePublished - 22 Jan 2016

Keywords

  • colorimetric probe
  • cyanine
  • fluorescent probe
  • nucleophilic addition
  • pH sensors

Fingerprint

Dive into the research topics of 'A Dual Colorimetric/Fluorescence System for Determining pH Based on the Nucleophilic Addition Reaction of an o-Hydroxymerocyanine Dye'. Together they form a unique fingerprint.

Cite this