A DNA strand-nicking principle of a higher plant, Caesalpinia sappan

Woongchon Mar; Hyun Tai Lee; Kang Hoon Je; Hye Young Choi; Eun Kyoung Seo

doi:10.1007/bf02976661

A DNA strand-nicking principle of a higher plant, Caesalpinia sappan

Woongchon Mar, Hyun Tai Lee, Kang Hoon Je, Hye Young Choi, Eun Kyoung Seo

Department of Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

To find anticancer agents from higher plants, DNA strand-scission assay method was employed for bioassay-guided fractionation as well as for screening the crude extracts. During the screening, an ethyl acetate extracts of the heartwood of Caesalpinia sappan L. (Leguminosae) exhibited potent DNA strand-scission activity. Therefore, the ethyl acetate extracts of the dried heartwood of C. sappan was subjected to the bioassay-guided fractionation, which led to the isolation of a known compound, brazilin (1) as the active constituent. In addition, caesalpine J (2) was also isolated as an inactive constituent.

Original language	English
Pages (from-to)	147-150
Number of pages	4
Journal	Archives of Pharmacal Research
Volume	26
Issue number	2
DOIs	https://doi.org/10.1007/bf02976661
State	Published - 28 Feb 2003

Bibliographical note

Funding Information:
This investigation was supported by a grant (PF002201-02) from the Plant Diversity Research Center of the 21st Frontier Research Program funded by the Korean Ministry of Science and Technology.

Keywords

Brazilin
Caesalpinia sappan
DNA strand-scission

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10.1007/bf02976661

Cite this

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title = "A DNA strand-nicking principle of a higher plant, Caesalpinia sappan",

abstract = "To find anticancer agents from higher plants, DNA strand-scission assay method was employed for bioassay-guided fractionation as well as for screening the crude extracts. During the screening, an ethyl acetate extracts of the heartwood of Caesalpinia sappan L. (Leguminosae) exhibited potent DNA strand-scission activity. Therefore, the ethyl acetate extracts of the dried heartwood of C. sappan was subjected to the bioassay-guided fractionation, which led to the isolation of a known compound, brazilin (1) as the active constituent. In addition, caesalpine J (2) was also isolated as an inactive constituent.",

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note = "Funding Information: This investigation was supported by a grant (PF002201-02) from the Plant Diversity Research Center of the 21st Frontier Research Program funded by the Korean Ministry of Science and Technology.",

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AU - Seo, Eun Kyoung

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N2 - To find anticancer agents from higher plants, DNA strand-scission assay method was employed for bioassay-guided fractionation as well as for screening the crude extracts. During the screening, an ethyl acetate extracts of the heartwood of Caesalpinia sappan L. (Leguminosae) exhibited potent DNA strand-scission activity. Therefore, the ethyl acetate extracts of the dried heartwood of C. sappan was subjected to the bioassay-guided fractionation, which led to the isolation of a known compound, brazilin (1) as the active constituent. In addition, caesalpine J (2) was also isolated as an inactive constituent.

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A DNA strand-nicking principle of a higher plant, Caesalpinia sappan

Abstract

Bibliographical note

Keywords

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