Abstract
We constructed a disposable bacterial lysis cartridge (BLC) suitable for an in situ pathogen detection system. It had an in-built micro corona discharge based ozone generator that provided ozone for cell lysis. Using a custom sample handling platform, its performance was evaluated with a Gram-positive bacterium of Bacillus subtilis. It was capable of achieving a similar degree of lysis as a commercial ultrasonic dismembrator with a P-1 microprobe in 10 min at an air pump flow rate of 29.4 ml min-1 and an ozone generator operating voltage of 1600 V. The lysing duration could be significantly reduced to 5 min by increasing the air pump flow rate and the ozone generator operating voltage as well as by the addition of sodium dodecyl sulfate (SDS).
| Original language | English |
|---|---|
| Pages (from-to) | 7776-7783 |
| Number of pages | 8 |
| Journal | Analyst |
| Volume | 140 |
| Issue number | 22 |
| DOIs | |
| State | Published - 21 Nov 2015 |
Bibliographical note
Publisher Copyright:© 2015 The Royal Society of Chemistry.