A coumarin-based reversible two-photon fluorescence probe for imaging glutathione near N-methyl-d-aspartate (NMDA) receptors

Nahyun Kwon, Chang Su Lim, Dayoung Lee, Gyeongju Ko, Jeongsun Ha, Moonyeon Cho, K. M.K. Swamy, Eun Young Lee, Dong Joon Lee, Sang Jip Nam, Xin Zhou, Hwan Myung Kim, Juyoung Yoon

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Glutathione (GSH) is known to play a key role in the modulation of the redox environment in N-methyl-d-aspartate (NMDA) receptors. Coumarin derivative 1 bearing cyanoacrylamide and ifenprodil moieties was synthesized and reported to monitor GSH near NMDA receptors. The cyanoacrylamide moiety allows probe 1 to monitor GSH reversibly at pH 7.4 and the ifenprodil group acts as a directing group for NMDA receptors. Two-photon fluorescence microscopy allows probe 1 to successfully sense endogenous GSH in neuronal cells and hippocampal tissues with excitation at 750 nm. Furthermore, the addition of H2O2 and GSH induced a decrease and an increase in fluorescence emission. Probe 1 can serve as a potential practical imaging tool to get important information on GSH in the brain.

Original languageEnglish
Pages (from-to)3633-3636
Number of pages4
JournalChemical Communications
Volume58
Issue number22
DOIs
StatePublished - 24 Feb 2022

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