In this study, 2-pyrone-4,6-dicarboxylic acid (PDC), a valuable monomer for bio-degradable plastics, was synthesised using terephthalic acid (TPA) derived from PET waste by a comprehensive chemo-microbial hybrid process. Depolymerisation of PET into TPA was performedviamicrowave-assisted hydrolysis using biomass-derived SiO2catalysts with thiol functionalisation. The increased PET hydrolysis efficiency was investigated by reaction kinetics studies. The conversion of TPA obtained by depolymerisation of PET waste into PDC was achieved using two recombinantEscherichia colistrains expressingtphAabcandtphBgenes for TPA degradation into protocatechuic acid (PCA) through 3,4-dihydroxy-cyclohexa-1,5-diene-1,4-dicarboxylic acid (DCD) (E. coliPCA strain) and usingligABCgenes to convert PCA to PDC through 4-carboxy-2-hydroxymuconate semialdehyde (CHMS) (E. coliPDCPCAstrain). Based on the high chemical depolymerisation of PET waste into TPA (over 97% efficiency), the double-catalyst system composed of theE. coliPCA strains with TPA degradation module and PDC synthesis module enables PET waste to be used in the PDC production platform with 96.08% of the overall efficiency.