A Biocompatible Probe for the Detection of Neutrophil Elastase Free from the Interference of Structural Changes and Its Application to Ratiometric Photoacoustic Imaging In Vivo

Ling Huang, Wanting Su, Lin Zhu, Jiangfeng Li, Wei Quan, Juyoung Yoon, Weiying Lin

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Neutrophil elastase (NE) plays a key role in chronic inflammation and acute responses to infection and injury. Effective disease interventions thus call for precise identification of NE to aid the clinical treatment of such diseases. However, the detection process suffers from the interference of structural changes of NE. Herein, we introduce a molecular probe with high biocompatibility to overcome the interference, which was achieved by combining theoretical calculations and experimental studies, that permits highly specific and sensitive detection of NE in cells and in vivo. The upregulated NE accumulation was specifically measured in inflammation by ratiometric photoacoustic and near-infrared fluorescence imaging, providing a new method for developing more specific fluorogenic probes for other enzymes.

Original languageEnglish
Article numbere202217508
JournalAngewandte Chemie - International Edition
Volume62
Issue number9
DOIs
StatePublished - 20 Feb 2023

Bibliographical note

Publisher Copyright:
© 2022 Wiley-VCH GmbH.

Keywords

  • Biocompatible Probes
  • Near-Infrared Fluorescence Imaging
  • Neutrophil Elastase
  • Photoacoustic Imaging

Fingerprint

Dive into the research topics of 'A Biocompatible Probe for the Detection of Neutrophil Elastase Free from the Interference of Structural Changes and Its Application to Ratiometric Photoacoustic Imaging In Vivo'. Together they form a unique fingerprint.

Cite this