TY - JOUR
T1 - A Biocompatible Probe for the Detection of Neutrophil Elastase Free from the Interference of Structural Changes and Its Application to Ratiometric Photoacoustic Imaging In Vivo
AU - Huang, Ling
AU - Su, Wanting
AU - Zhu, Lin
AU - Li, Jiangfeng
AU - Quan, Wei
AU - Yoon, Juyoung
AU - Lin, Weiying
N1 - Publisher Copyright:
© 2022 Wiley-VCH GmbH.
PY - 2023/2/20
Y1 - 2023/2/20
N2 - Neutrophil elastase (NE) plays a key role in chronic inflammation and acute responses to infection and injury. Effective disease interventions thus call for precise identification of NE to aid the clinical treatment of such diseases. However, the detection process suffers from the interference of structural changes of NE. Herein, we introduce a molecular probe with high biocompatibility to overcome the interference, which was achieved by combining theoretical calculations and experimental studies, that permits highly specific and sensitive detection of NE in cells and in vivo. The upregulated NE accumulation was specifically measured in inflammation by ratiometric photoacoustic and near-infrared fluorescence imaging, providing a new method for developing more specific fluorogenic probes for other enzymes.
AB - Neutrophil elastase (NE) plays a key role in chronic inflammation and acute responses to infection and injury. Effective disease interventions thus call for precise identification of NE to aid the clinical treatment of such diseases. However, the detection process suffers from the interference of structural changes of NE. Herein, we introduce a molecular probe with high biocompatibility to overcome the interference, which was achieved by combining theoretical calculations and experimental studies, that permits highly specific and sensitive detection of NE in cells and in vivo. The upregulated NE accumulation was specifically measured in inflammation by ratiometric photoacoustic and near-infrared fluorescence imaging, providing a new method for developing more specific fluorogenic probes for other enzymes.
KW - Biocompatible Probes
KW - Near-Infrared Fluorescence Imaging
KW - Neutrophil Elastase
KW - Photoacoustic Imaging
UR - http://www.scopus.com/inward/record.url?scp=85146463305&partnerID=8YFLogxK
U2 - 10.1002/anie.202217508
DO - 10.1002/anie.202217508
M3 - Article
C2 - 36578174
AN - SCOPUS:85146463305
SN - 1433-7851
VL - 62
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
IS - 9
M1 - e202217508
ER -