益生菌-海带复合发酵液对肥胖大鼠肠道菌群的影响及抗肥胖效果

Aim; To explore the regulatory meehanism of the probiotics and sea-tent fermentation liquid on gut mierobes. MethodS;The obesity rat model indueed by high-fat diet was established and was randomly alloeated into the obesity eontrol group[high-fat diet + general drinking water],the probiotic fermentation group [high-fat feed + 10% (V/V) probiotic in general drinking water], and the probiotic and sea-tent fermentation group [high-fat diet + 10% (V/V) probiotic and sea-tent fermentation liquid in general drinking water], and normal group (normal diet + general drinking water),with 10 rats in eaeh group. After 8 weeks of dietary intervention, the weight ehanges of rats before and after the experiment, the water intake and daily average food intake of rats one day before exeeution were observed. After sacrificing, the contents of the Colon were taken,the pyrophosphate sequencing method was used to identify intestinal mierobial flora, and the contents of butyric acid and propionic acid in the intestine were determined by gas ehromatography-mass spectrometry. In addition,the differences in the intestinal flora and short-chain fatty acid production in each group were compared. Results; Compared with the other 3 groups,the weight gain,and water intake of obese rats in the probiotic and sea-tent fermentation group deereased (P < 0. 05). Compared with the obese eontrol group, the abundanoe of Firmicutes deereased, the abundanoe of Bacteroides increased in the probiotic and sea-tent fermentation group (P < 0. 05). Compared with the obese eontrol group, the ratio of Firmicutes/ Bacteroides in the intestinal traet of the probiotic and sea-tent fermentation group and the probiotic fermentation group deereased^ < 0. 05). Compared with the probiotic fermentation group, the ratio of Firmicutes/Bacteroides deereased in probiotic and sea-tent fermentation group (P < 0. 05). Compared with the obese eontrol group, the abundance of microbial related to obesity in the intestinal tract of rats in the probiotic and sea-tent fermentation liquid group deereased; the abundance of anti-obesity microbial increased (P < 0. 05). Compared with the other 3 groups,the contents of butyric acid and propionic acid in the intestinal tract of the probiotic and sea-tent fermentation group increased(P < 0. 05). Conclusion; The combina-tion of probiotics and sea-tent can significantly reduce the proportion of Firmicutes/Bacteroides, enhance the production of short-chain fatty aeids, and improve intestinal health,thus has good anti-obesity effect.