干酪乳杆菌山药复合发酵剂对溃疡性结肠炎小鼠肠道菌群的调节 及其抗炎作用

Objective　To explore the regulation effects of Lactobacillus casei-yam compound starter on intestinal flora and its anti-inflammatory in mice with ulcerative colitis (UC).Methods　Lactobacillus casei (SJP6841L2) and yam powder were mixed and fermented to produce Lactobacillus casei-yam compound starter.A total of 60 mice were randomly divided into blank control group,UC model group,yam control group,low-dose compound starter group,medium-dose compound starter group and high-dose compound starter group,with 10 mice in each group.The mice in the UC model group,yam control group,low-dose compound starter group,medium-dose compound starter group and high-dose compound starter group were induced by 2,4,6-trinitrobenzene sulfonic acid to prepare UC model;the mice in the normal control group were injected with volume fraction 50% ethanol through anus.After successful modeling,the mice in the normal control group and UC model group were administrated normal saline (10 mL·kg-1) by oral gavage every day;the mice in the yam control group were administrated 200 mg·kg-1 non-fermented yam preparation by oral gavage every day;and the mice in the low-dose compound starter group,the medium-dose compound starter group and the high-dose compound starter group were administrated 50,100 and 200 mg·kg-1 Lactobacillus casei-yam compound starter by oral gavage every day,respectively,lasted for 10 days.After treatment for 10 days,the general symptoms including mucopurulent bloody stool and diarrhea of mice in each group were observed;the mice in each group were killed,the colon was taken,and the length,thickness and weight of the colon were measured,the edema of the colon tissue was observed;the pathological changes of the colon tissue of mice in each group was observed by hematoxylin-eosin staining,the activity of MPO in colon tissue of mice in each group was detected by ultraviolet spectrophotometry,and the expressions of IL-8,TNF-α,HO-1 and NF-κB in colon tissue of mice in each group were detected by enzyme-linked immunosorbent assay,the expressions of intestinal up-regulated seal protein 1 (claudin 1) and ZO-1 in colon tissue of mice in each group were detected by Western blot method,the number of bifidobacteria,lactobacilli,bacteroides and enterobacteria in intestinal tract of colon were detected by bacterial physiological and biochemical identification method,the levels of acetic acid,propionic acid and butyric acid in intestinal tract of colon of mice in each group were detected by gas chromatography method.